+- +-

+-User

Welcome, Guest.
Please login or register.
 
 
 
Forgot your password?

+-Stats ezBlock

Members
Total Members: 75
Latest: Farshad
New This Month: 0
New This Week: 0
New Today: 0
Stats
Total Posts: 104
Total Topics: 75
Most Online Today: 2
Most Online Ever: 22
(May 28, 2019, 04:37:54 am)
Users Online
Members: 0
Guests: 1
Total: 1

Author Topic: Corneal repair by iPSCs and amphiphatic chitosan hydrogel  (Read 421 times)

0 Members and 0 Guests are viewing this topic.

SSamani

  • Board Moderator
  • Newbie
  • *
  • Posts: 39
Corneal repair by iPSCs and amphiphatic chitosan hydrogel
« on: February 21, 2015, 11:46:21 pm »
Corneal repair by human corneal keratocyte-reprogrammed iPSCs and amphiphatic carboxymethyl-hexanoyl chitosan hydrogel

Biomaterials 33 (2012) 8003-8016
Abstract
Induced pluripotent stem cells (iPSCs) have promising potential in regenerative medicine, but whether iPSCs can promote corneal reconstruction remains undetermined. In this study, we successfully reprogrammed human corneal keratocytes into iPSCs. To prevent feeder cell contamination, these iPSCs were cultured onto a serum- and feeder-free system in which they remained stable through 30 passages and showed ESC-like pluripotent property. To investigate the availability of iPSCs as bioengineered substitutes in corneal repair, we developed a thermo-gelling injectable amphiphatic carboxymethyl-hexanoyl chitosan (CHC) nanoscale hydrogel and found that such gel increased the viability and CD44+proportion of iPSCs, and maintained their stem-cell like gene expression, in the presence of culture media. Combined treatment of iPSC with CHC hydrogel (iPSC/CHC hydrogel) facilitated wound healing in surgical abrasion-injured corneas. In severe corneal damage induced by alkaline, iPSC/CHC hydrogel enhanced corneal reconstruction by downregulating oxidative stress and recruiting endogenous epithelial cells to restore corneal epithelial thickness. Therefore, we demonstrated that these human keratocyte-reprogrammed iPSCs, when combined with CHC hydrogel, can be used as a rapid delivery system to efficiently enhance corneal wound healing. In addition, iPSCs reprogrammed from corneal surgical residues may serve as an alternative cell source for personalized therapies for human corneal damage.
« Last Edit: April 21, 2015, 09:49:00 pm by SSamani »
The true sign of intelligence is not knowledge but imagination. Albert Einstein

Share on Facebook Share on Twitter


 

Recent Posts ezBlock

Re: سایت انجمن by nourani
February 21, 2017, 10:10:10 am

سایت انجمن by nourani
February 21, 2017, 10:08:58 am

دومین کنگره سراسری پیشرفت‌های مهندسی باف by nourani
November 06, 2015, 10:53:12 pm

2015 TERMIS World Congress by nourani
September 03, 2015, 05:03:47 am

Dr. Zohreh Bagher The first PhD in Tissue Engineering graduated from IUMS by nourani
July 01, 2015, 04:27:43 pm

Journal of Applied Tissue Engineering was published by nourani
June 14, 2015, 09:30:06 pm

Antibacterial Hydroxyapatite (A review) by SSamani
May 02, 2015, 02:36:54 pm

Bone grafting is a big business by nourani
April 22, 2015, 09:38:11 pm

Controlled Dual Release of Functional Molecules by SSamani
April 20, 2015, 11:53:23 pm

Hyaluronic Acid Hydrogel: A Favourable Niche for Neural Stem Cells by SSamani
March 28, 2015, 01:59:53 am